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Biomarkers for DNA oxidation. oxidative stress
New 8-OHdG Check ELISA
8-oxo-dG/8-hydroxy-2'-deoxyguanosine
Suitable for assessment of oxidative stress using urine samples. For research use only.
About 8-hydroxy-2'-deoxyguanosine (8-OHdG):
8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.
This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use 'Highly Sensitive 8-OHdG Check ELISA'.
Specifications
Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Measuring range: 0.5 to 200 ng/mL
Format: 96 wells (18 samples in triple assays)
Applications: Urine from human and animals.
For serum and tissue samples, Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended.
Storage: Store at 4 - 10°C (don't freeze).
Expiry: 12 months after the day of manufacturing.
Required but not provided: Micropipet and chip (100 micro L, 1000 micro L).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm).
Content of this kit
8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type) 1 plate
Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial
Primary Antibody Solution   1 vial (6mL)
Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial
Secondary Antibody Solution:   1 vial (12mL)
Chromatic Solution: 3,3',5,5'-tetramethylbenzidine 1 vial (0.25mL)
Diluting Solution: H2O2 containing buffer. 1 vial (12mL)
Washing Solution(5x):   2 vials (26mL x 2)
Reaction Terminating Solution: 1M Phosphoric acid. 1 vial (12mL)
Standard 8-OHdG Solution: Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL) 1 vial each
Plate Seal:   2 sheets
References
1) H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]
2) R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]
3) S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.]
4) Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ]
5) Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T, Yoshimura M, Ogawa H: Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 95(4), p514-517 (2005)
[ Measurement of 8-OHdG in human urine. ]
6) Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J: The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5-6),p543-549(2005)
[ Study about exercise, antioxidant supplements and urinary 8-OHdG. ]
7) Someya T, Kaneko K, Yamada T, Yamashiro Y: Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol 20(10),p1430-1434(2005)
[ Effect of antioxidants to 8-OHdG in rat urine. ]
8) Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci 250(1-2),p159-161(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]
Product name Code Assay range Format Application
New 8-OHdG Check KOG-200SE 0.5-200 ng/mL 96 wells Urine and animal serum.

Made in Japan.

Related products:    Highly sensitive 8-OHdG Check ELISA    Anti 8-OHdG monoclonal antibody
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