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Trace elenets assay kits.
Traceelements Iron colorimetric assay kit
Easy to use. For cell lysates, serum, plasma and wide variety of biological samples.
[ Product Description ]
Pre-preatment free!
Species independent!
No toxic substance!
Applicable to microplate reader!


Iron is a mineral which binds many proteins and enzymes, and plays important roles in living bodies. MC-Reagent Fe Assay Kit provides a simple and convenient tool for the determination of Ferrous and/or Ferric ion in a variety of biological samples such as cell lysates, tissue homogenates, serum, plasma, saliva, urine, tissue extract, plant extract, hair and food extract.
[ Performance ]
1. Sensitivity: O.D. at 200 µg/dL standard will be 0.08 - 0.25
2. Accuracy: ±10% of known concentration
3. Intra assay variation: C.V. is less than 5%
4. Assay range: 5 - 1,000 µg/dL
 
Not be affected by following substances.
1. Hemoglobin: up to 70 mg/dL
2. Bilirubin: up to 90 mg/dL
This assay will be interfered by EDTA.


Good liearity for full assay range

Good correlation with ICP

Easy to apply to tissue samples
[ Specifications ]
Assay principle: Ferrozine method (Colorimetric assay at 560nm)
Specifity: Specific to Fe2+ and Fe3+
Measuring range: 5 - 1,000 µg/dL
Sample volume: 40 µL
Assay conditions: 10 minutes at room temperature (25 - 37 °C)
Applications: Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Metal chelatos, for example EDTA may interfere with this assay.
Storage temperature: 2 - 8°C (don't freeze).
Expiry date: 1 year after the day of manufacturing.
Required but not provided: 96-well plate
Pipeting devices such as 8-channel (50-200 µL) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microtiter plate reader (measuring wavelength between 540 and 580nm. 560nm is recommended.)
[ Content of this kit ]
R-A Buffer (ready to use): 1 x 40 mL
R-R Chelate color (ready to use): 1 x1.6 mL
Iron standard 200 µg/dL (ready to use): 1 x 8 mL
[ References: Assay examples ]
1) Y Ikeda, S Tajima, YI-Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya and T Tamaki:
Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes.
PLoS ONE 7(7),e40465(2012) doi: 10.1371/journal.pone.0040465
2) Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T:
FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach.
Free Radical Biology and Medicine 52(6),p1003-1010(2012) doi: 10.1016/j.freeradbiomed.2011.12.011.
3) M Nakaya, M Tajima, H Kosako, T Nakaya, A Hashimoto, K Watari, H Nishihara, M Ohba, S Komiya, N Tani, M Nishida, H Taniguchi, Y Sato, M Matsumoto, M Tsuda, M Kuroda, K Inoue and H Kurose:
GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
Nature Communications 4, 1532 (2013) doi:10.1038/ncomms2540
4) Y Ikeda, H Enomoto, S Tajima, YI Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya, T Tamaki:
Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice.
American Journal of Physiology - RenalPhysiology 304:F1028-F1036(2013) DOI:10.1152/ajprenal.00473.2012
5) Y Ikeda, I Ozono, S Tajima, M Imao, Y Horinouchi, YI Ishizawa, Y Kihira, L Miyamoto, K Ishizawa, K Tsuchiya, T Tamaki:
Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction.
PLoS ONE 9(2), e89355(2014) doi:10.1371/journal.pone.0089355
Product name Catalog# Assay range Size Price
MC Reagent Fe Assay Kit (Ferrozine) CFE-005 5-1,000 µg/dL 100 tests US$330 / €254

Made in Japan.

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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

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