Home > Selection Guide for Oxidative Stress Markers [ Print page ] Rev.161219
Selection guide for oxidative stress markers.  
Type of
marker
Item Spec. Applications Remark
Urine Serum Cells
DNA
oxidation
marker
8-OHdG
New 8-OHdG Check ELISA
(Code. KOG-200SE), ELISA: 450nm, 96 wells

Highly Sensitive 8-OHdG Check ELISA
(Code. KOG-HS10E), ELISA: 450nm, 96 wells

Anti 8-OHdG monoclonal antibody (N45.1)
(Code: MOG-020P / MOG-100P)

 
Assay range:
0.5 - 200 ng/mL

Assay range:
0.125-10 ng/mL

20 µg /
100 µg of IgG

 
OK


OK


-


 
OK


OK


-


 
-


OK


OK


 
Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE)

Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E)
Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG).
Anti Thymidine Glycol (TG) antibody
(Code: MTG-100P) Clone 2E8
100 µg of IgG - - OK DNA-specific oxidative stress marker. Specific to TG in DNA polymer.
Lipid
oxidation
marker
HEL (13HPODE-Lys adduct)
Hexanoyl-Lysine (HEL) ELISA kit
(Code: KHL-700E), ELISA: 450nm, 96 wells

Anti Hexanoyl-Lysine (HEL) antibody
(Code: MHL-021P) Clone 5F12
 
Assay range:
2 - 700 nmol/L

20 µg of IgG

 
OK


-

 
OK


-

 
OK


OK

Nε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells.
4-HNE
Anti 4-HNE monoclonal antibody (HNEJ2)
(Code: MHN-020P / MHN-100P)
 
20 µg /
100 µg of IgG
 
-
 
 
-
 
 
OK
 
4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins.
MDA
Anti MDA monoclonal antibody
(Code: MMD-030n) Clone 1F83

Malondialdehyde assay kit (TBARS)
(Code: KMD-008W) Colorimetric: 400-700nm scan
 
30 µg of IgG


Assay range:
0.08 - 10 µmol/L
 
-


OK
 
 
-


OK
 
 
OK


OK
 
Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine).
By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay.
Isoprostane
Urinary isoprostane ELISA
(Code: KIP-050) ELISA: 450nm, 96wells

Serum isoprostane ELISA
(Code: KIP-001W) ELISA: 450nm, 96wells
 
Assay range:
0.05 - 100 ng/mL

Assay range:
0.05 - 10 ng/mL
 
OK


-
 
 
-


OK
 
 
-


OK
 
 
15-Isoprostane F2t (also known as 8-epi-PGF or 8-iso-PGF) is produced by peroxidation of lipoproteins.
For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed.
Anti Acrolein (ACR) antibody
(Code: MAR-020n / MAR-100n) Clone 5F6
20 µg /
100 µg of IgG
- - OK Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine).
Anti 4-hydroxy-2-hexenal (4-HHE) antibody
(Code: MHH-030n) Clone HHE53
30 µg of IgG - - OK Specific to HHE-histidine Michael adduct (HHE-His).
Anti Crotonaldehyde (CRA) antibody
(Code: MCA-030n) Clone 82D3
30 µg of IgG - - OK Specific to CRA-protein aduucts.
Anti Methylglyoxal (MG) antibody
(Code: MMG-030n) Clone 3C
30 µg of IgG - - OK Specific to MG-protein adduct (argpyrimidine).
Anti 7-ketocholesterol (7-KC) antibody
(Code: MKC-020n) Clone 35A
20 µg of IgG - - OK Biomarker of cholesterol oxidation. Applicable for frozen tissue only.
Mitochondria
Lipid peroxide probe (mitochondria) [NEW]
(Code: PLP-466J)
 
5 µg x3
 
 
-
 
 
-
 
 
OK
 
Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells.
Lipid peroxide probe (DPPP) [NEW]
(Code: PLP-350J)
10 mg - OK OK Fluorescence probe for lipid peroxides. For HPLC application.
Protein
oxidation
marker
Dityrosne (Tyr dimer)
Dityrosine (DT) ELISA [NEW]
(Code: KDT-010E), ELISA: 450nm, 96 wells

Anti Dityrosine (DT) antibody
(Code: MDT-020P) Clone 1C3
 
Assay range:
0.05-12 µmol/L

20 µg of IgG
 
 
OK


-
 
 
OK


-
 
 
-


OK
 
 
New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours.

Anti Dibromo-Tyrosine (DiBrY) antibody
(Code: MBY-020P) Clone 3A5
20 µg of IgG - - OK Inflammation-related protein oxidation marker.
NO-modification
Anti Nitrotryptophan (NW) antibody [NEW]
(Code: MNW-020P) Clone 117C
 
20 µg of IgG
 
 
-
 
 
-
 
 
OK
 
A novel nitrosative stress marker derived from peroxynitrite and tryptophan.
Nitrotyrosine ELISA
(Code: KNT-002W) 96 wells
2 - 1500 nmol/L - OK Δ
Anti-
oxidant

assay
Total Antioxidant
Test kit for Potential Antioxidant(PAO), 96 wells
(Code: KPA-050) Colorimetric: 480-492 nm

TAC Peroxyl assay,  96 wells
(Code: KPR-005W) Luminescence
 
Assay range:
21.9- 4378 µmol/L

Assay range:
0.5กม30 µmol/mL
 
-


-
 
 
OK


OK
 
 
OK


OK
 
 
Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants.
KPR-005W can detect peroxyl radicals(ROO·) scavenging activity.
GSH
Total Glutathione assay kit 100 tests [NEW]
(Code: KGS-419J) Colorimetric: 405-415 nm
 
Assay range:
5-100 µmol/L
 
-
 
 
-
 
 
OK
 
The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria.
Enzyme
assay
SOD
SOD activity assay kit (WST-1 method)  [NEW]
(Code: KSD-311J) Colorimetric: 450 nm, 500 tests

SOD activity assay kit (Hematoxylin method)
(Code: KSD-005W) Colorimetric: 560 nm, 96 wells

Cu/Zn-SOD ELISA (Human SOD1)
(Code: KSE-040W) ELISA: 450 nm, 96 wells

Mn-SOD ELISA (Human SOD2)
(Code: KSG-025W) ELISA: 450 nm, 96 wells

ec-SOD ELISA (Human SOD3)
(Code: KSF-002W) ELISA: 450 nm, 96 wells
 



Assay range:
5-60 U/mL

Assay range:
0.04 - 5 ng/mL

Assay range:
25 - ng/mL

Assay range:
2 - 168 ng/mL
 
-


-


-


-


-
 
 
OK


Eryth-
rocyte

OK


-


-
 
 
OK


OK


OK


OK


OK
 
 
Can be applied to all animals, plants and bacteria.




SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum.
Mn-SOD(SOD2) is detectable in Mitochondria.



CAT
Catalase activity assay kit 96 wells
(Code: KCA-003W) UV: 240 nm
 
Assay range:
6-150 U/mL
 
-
 
Eryth-
rocyte
 
OK
 
MPO activity assay kit
(Code: KMP-008W) Colorimetric: 412 nm
96 microplate assays or 30 cubette assays
Assay range:
8- 400 U/mL
- OK OK
GSH metabolism
Glutathion Peroxidase (GPx) assay kit 192 wells
(Code: KGX-005W) Colorimetric: 340 nm

Glutathion Reductase (GR) assay kit 192 wells
(Code: KGR-015W) Colorimetric: 340 nm
 
Assay range:
5-60 mU/mL

Assay range:
1.5-20 mU/mL
 
-


-
 
 
Blood


Blood
 
 
OK


OK
 
 
Redox
regulation
markers
Thioredoxin
Thioredoxin I ELISA (human) 96 wells
(Code: KRX-004W) ELISA: 450 nm
 
Assay range:
0.4- 25 ng/mL
 
-
 
 
OK
 
 
Δ
 
 
Thioredoxin reductase I ELISA (human) 96 wells
(Code: KRR-004W) ELISA: 450 nm
Assay range:
0.4- 25 ng/mL
- OK Δ  
Peroxiredoxin I ELISA (human) 96 wells
(Code: KPX-005W) ELISA: 450 nm
Assay range:
0.5 - 32 ng/mL
- OK Δ  
Peroxiredoxin III ELISA (human) 96 wells
(Code: KPZ-002W) ELISA: 450 nm
Assay range:
2 - 32 ng/mL
- OK Δ  
Trace
elements

assay
Fe
Iron assay kit (Ferrozine) 100 tests
(Code: CFE-005) Colorimetric: 540-580 nm

Iron assay kit (Nitroso-PSAP) 100 tests
(Code: CFE-010) Colorimetric: 740-760 nm

UIBC assay kit (Bathophenanthroline) 100 tests
(Code: CBC-800) Colorimetric: 540-550 nm
 
Assay range:
5-1,000 µg/dL

Assay range:
10-1,000 µg/dL

Assay range:
10-800 µg/dL
 
OK


OK


-
 
 
OK


OK


OK
 
 
OK


OK


-
 
 
Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.

Detection of unsaturated iron binding capacity(UIBC). Species independent.
Cu
Copper assay kit (3,5-DiBr-PAESA) 100 tests
(Code: CCU-400) Colorimetric: 570-590 nm
 
Assay range:
3-400 µg/dL
 
OK
 
 
OK
 
 
OK
 
Can detect total (free and ceruloplasmin - bond) copper. Also applicable to extractes from tissues, plants and hair.
Zn
Zink assay kit (5-Br-PAPS) 
(Code: CZN-001) Colorimetric: 550-580 nm
 
Assay range:
4-1,000 µg/dL
 
OK
 
 
OK
 
 
OK
 
Can detect total (free and protein - bond) zinc. Also applicable to extractes from tissues, plants and hair.
Ca
Calcium assay kit (Chlorophosphonazo III)
(Code: CCA-020) Colorimetric: 680-700 nm, 200 tests

Calcium assay kit (o-Cresolphtalein-Complexone)
(Code: CCA-030) Colorimetric: 560-590 nm, 200 tests
 
Assay range:
0.05-20 mg/dL

Assay range:
0.2-30 mg/dL
 
OK


OK
 
 
OK


OK
 
 
OK


OK
 
Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.
Mg
Magnesium assay kit (Xyridil Blue-I) 100 tests
(Code: CMG-035) Colorimetric: 660 nm
 
Assay range:
0.05-3.5 mg/dL
 
OK
 
 
OK
 
 
OK
 
Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.
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List of oxidative stress assay kits and antibodies
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