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Oxidative Stress
Rev.160826 |
Anti MDA monoclonal antibody
(Anti-malondialdehyde: clone 1F83) |
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| About malondialdehyde (MDA) |
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Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation.
MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of
numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative.
This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.
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| Specifications |
| Clone #: |
1F83 |
| Antigen: |
MDA-modified keyhole-lympet hemocyanine. |
| Form: |
Frozen (100 µg/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA).
Purified by Protein-A. |
| Application: |
Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 µg/mL on paraformaldehyde fixed tissue. |
| Specificity: |
MSpecific for MDA-modified protein (especially DHP-lysine). |
| Subclass: |
Mouse IgG2a(lambda) |
| Storage: |
Less than -20°C |
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| Product name |
Code |
Content |
| Anti Malondialdehyde (MDA) monoclonal antibody |
MMD-030n |
30 µg IgG |
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Manufactured by NOF corporation, Japan. |
| FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use. |
| Procedure |
| 1) |
Paraffin sections were deparaffinized and rehydrated. |
| 2) |
Sections were quenched for several minutes with 3% hydrogen peroxide,
rinsed in PBS, pretreated for 30 min with 3% nonimmune animal serum in PBS. |
| 3) |
Sections were incubated overnight at 4°C with a antibody at a
dilution of 1 µg/mL. |
| 4) |
Antibody binding was visualized by the avidin-biotin-immuno
peroxidase complex method using the Vectastain ABC kit (Vector, Burlingame, CA) according to the manufacturer's instructions. |
| 5) |
3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen. |
| 6) |
Hematoxylin or Eosin was used as the counter stain. |
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|
| 1) |
Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
J.Lipid Res. 42, p1187-1196 (2001) |
| 2) |
Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.
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| FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use. |
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