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Oxidative / Nitrosative Stress
Rev.151226 |
Anti Nitrotryptophan
monoclonal antibody |
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| A novel biomarker for protein oxidation/nitration: |
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Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis,
inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent
biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide
(O2-). Peroxinitrite can induce nitration of aromatic amino acids such as
Tyr, Trp, Phe and His, which results in modulation of protein functions.
To date, 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major
ONOO--modified amino acids, and growing evidences
suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress.
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An anti-6-nitrotryptophan monoclonal antibody has been developed by Prof. Yamakura at Juntendo University,
which is suitable for immunohistochemistry, western blotting and ELISA. This antibody may provide a powerful
tool for the investigation of nitrosative stress inside living bodies.
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| Specifications |
| Antigen: |
6-nitrotryptophan |
| Form: |
Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG. |
| Subclass: |
Mouse IgG1(kappa), clone 117C. Protein A purified. |
| Specificity: |
Specific for 6-nitrotryptophan |
| Application: |
Immunohistochemistry, western blotting and ELISA |
| Storage: |
Less than -20°C |
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| Reference |
| 1) |
Ikeda K, Yukihiro Hiraoka B, Iwai H, Matsumoto T, Mineki R, Taka H,
Takamori K, Ogawa H, Yamakura F:
Detection of 6-nitrotryptophan in proteins by Western blot analysis and its application for peroxynitrite-treated PC12
cells.
Nitric Oxide 16(1):18-28(2007) |
| 2) |
Degendorfer G, Chuang CY, Hammer A, Malle E, Davies MJ.
Peroxynitrous acid induces structural and functional modifications to basement membranes and its key component, laminin.
Free Radic Biol Med 89,p721-733(2015). doi: 10.1016/j.freeradbiomed.2015.09.018.
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| Product name |
Code |
Content |
| Anti nitrotryptophan (NW) monoclonal antibody |
MNW-020P |
20 µg IgG |
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| FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use. |
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