Home > Products > Antibodies > Anti 4-HNE antibody [ Print page ] Rev.090316
Biomarkers for lipid oxidation. oxidative stress
Anti 4-HNE monoclonal antobody (HNE-J2)
Suitable for immunohistocheistry and western blotting. For research use only.
About 4-hydroxy-2-nonenal (4-HNE)

Structure of 4-hydroxy-2-nonenal
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.
High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)
Specifications
Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 µg or 100 µg of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature.
References
Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 µg of IgG/ vial
MHN-100P 100 µg of IgG/ vial

Made in Japan

Ordering Information
Instruction manual (PDF) Immunohistochemical procedure Technical information
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would causedby such faults, we will be exempt from responsibility.
Page top