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| Suitable for immunohistocheistry. For research use only. |
| About methylglyoxal (MG) |
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Methylglyoxal (MG), an endogenous metabolite
that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation
end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine.
This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.
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| Specifications |
| Clone #: |
3C |
| Antigen: |
MG-modified keyhole-lympet hemocyanine |
| Specificity: |
Specific for MG-modified protein (especially Argpyrimidine) |
| Subclass: |
Mouse IgG2a(kappa) |
| Form: |
Frozen (10mM PBS containing 0.1% NaN3 and 0.5% BSA, 100 µg/mL IgG.
Protein A purified. |
| Application: |
Immunohistochemistry；It is recommended that the antibody be tried at 0.5-1.0 µg/mL on
paraformaldehyde fixed tissue. |
| Storage: |
Less than -20°C |
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| Specificity of anti MG antibody. |
Immunohistochemical detection of MG adducts in atherosclerotic aorta.
Noriyuki Shibata et al. Tokyo Women’s medical University |
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| References |
| 1) |
Protein modification by a Maillard reaction intermediate methylglyoxal.
Immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo.
Uchida K, Khor OT, Oya T, Osawa T, Yasuda Y, Miyata T.
FEBS Lett. 410(2-3), p313-318. (1997) |
| 2) |
Methylglyoxal Modification of Protein -Chemical and Immunochemical
Characterization of Methylglyoxal-Arginine Adduct.
T.Oya, N.Hattori, Y.Mizuno, S.Miyata, S.Maeda, T.Osawa and K.Uchida.
J.Biological Chemistry, 274, 18492-18502 (1999) |
| 3) |
Advanced glycation and lipidoxidation of the peritoneal membrane:
respective roles of serum and peritoneal fluid reactive carbonyl compounds.
Miyata T, Horie K, Ueda Y, Fujita Y, Izuhara Y, Hirano H, Uchida K, Saito A, van Ypersele de Strihou C, Kurokawa K.
Kidney Int. 58(1), p425-435.(2000) |
| 4) |
Argpyrimidine, a blue fluorophore in human lens proteins: high levels
in brunescent cataractous lenses.
Padayatti PS, Ng AS, Uchida K, Glomb MA, Nagaraj RH.
Invest Ophthalmol Vis Sci. 42(6), p1299-1304. (2001) |
| 5) |
High concentrations of glucose induce synthesis of argpyrimidine in
retinal endothelial cells.
Padayatti PS, Jiang C, Glomb MA, Uchida K, Nagaraj RH.
Curr Eye Res. 23(2), p106-115. (2001) |
| 6) |
Curcumin Inhibits ROS Formation and Apoptosis in Methylglyoxal-
Treated Human Hepatoma G2 Cells.
WEN-HSIUNG CHAN, HSIN-JUNG WU and YAN-DER HSUUW.
Ann. N.Y. Acad. Sci. 1042: 372-378 (2005) |
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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility.
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