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| Suitable for assessment of oxidative stress in serum, tissue and cultured cells. For research use only. |
| About 8-hydroxy-2'-deoxyguanosine (8-OHdG): |
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8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical,
singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials.
New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is
highly specific for DNA damage, not cross react with RNA oxidation
products such as 8-hydroxy-guanine and 8-hydroxy-guanosine.
This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals.
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This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1)
which is highly specific for 8-OHdG.
We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum
, tissue and cultured cells.
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| Specifications |
| Assay principle: |
Competitive ELISA (detection: 450 nm) |
| Specifity: |
Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G,
6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea,
creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
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| Measuring range: |
0.125 to 10 ng/mL |
| Format: |
96 wells (18 samples in triple assays) |
| Applications: |
Urine, serum, tissue and cultured cells. |
| Storage: |
Store at 4 - 10°C (don't freeze). |
| Expiry: |
9 months after the day of manufacturing. |
| Required but not provided: |
Micropipet and chip (100 µL, 1000 µL).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm). |
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| Content of this kit |
| 8-OHdG Microtiter Plate: |
Precoated with 8-OHdG(12 X 8wells, split type) |
1 plate |
| Primary Antibody: |
Anti 8-OHdG antibody, powder. |
1 vial |
| Primary Antibody Solution |
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1 vial (6mL) |
| Secondary Antibody: |
HRP-anti mouse antibody, powder. |
1 vial |
| Secondary Antibody Solution: |
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1 vial (12mL) |
| Chromatic Solution: |
3,3',5,5'-tetramethylbenzidine |
1 vial (0.25mL) |
| Diluting Solution: |
H2O2 containing buffer. |
1 vial (12mL) |
| Washing Solution(5x): |
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2 vials (26mLx2) |
| Reaction Terminating Solution: |
1M Phosphoric acid. |
1 vial (12mL) |
| Standard 8-OHdG Solution: |
Purified 8-OHdG (0.125, 0.25, 0.5, 1, 4, 10 ng/mL) |
1 vial each |
| Plate Seal: |
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2 sheets |
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| References |
| 1) |
H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular
DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ] |
| 2) |
R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ] |
| 3) |
S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa:
Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1:
Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.] |
| 4) |
Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K:
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA.
Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ] |
| 5) |
M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert and J.Lunec:
Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage.
Biochem Biophys Res Commun 259, p374-378 (1999)
[ ELISA results show high correlation between those of HPLC-ECD when 8-OHdG in DNA samples are assessed. ] |
| 6) |
Ha Won Kim, Akira Murakami, Marshall V Williams and Hajime Ohigashi:
Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells.
Carcinogenesis 24(2), p235-241 (2003)
[ Detection of 8-OHdG in cultured cells. ] |
| 7) |
Watanabe E, Matsuda N, Shiga T, Kajimoto K, Ajiro Y, Kawarai H, Kasanuki H, Kawana M:
Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy.
J Card Fail. 2006 Sep;12(7):527-32.
[ Serum 8-OHdG in IDC patients are assessed. ] |
| 8) |
Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K:
Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated
with a peripheral marker of oxidative DNA damage.
J Neurol Sci. 250(1-2):159-61(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ] |
| 9) |
Reiko Nagasaka, Nobuaki Okamoto, Hideki Ushio:
Effects of caloric restriction on post-spawning death of ayu.
Exp Gerontol 40, p556-561(2005)
[ Detection of 8-OHdG in fish tissues (brain and liver). ] |
| 10) |
Yasuda M, Ide H, Furuya K, Yoshii T, Nishio K, Saito K, Isotani S, Kamiyama Y, Muto S, Horie S:
Salivary 8-OHdG: a useful biomarker for predicting severe ED and hypogonadism.
J Sex Med. 5(6),p1482-1491(2008)
[ 8-OHdG in saliva can be detected by 8-OHdG ELISA. ] |
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| Product name |
Code |
Assay range |
Application |
| Highly Sensitive 8-OHdG Check |
KOG-HS10E |
0.125 - 10 ng/mL |
Serum, plasma, tissue and other biological fluids. |
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Made in Japan. |
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would causedby such faults, we will be exempt from responsibility.
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