Oxidative Stress Marker Selection Guide: Japan Institute for the Control of Aging (JaICA)

Home > Selection Guide for Oxidative Stress Markers

Rev.161219

Type of marker	Item	Spec.	Applications			Remark
Type of marker	Item	Spec.	Urine	Serum	Cells	Remark
DNA oxidation marker	8-OHdG New 8-OHdG Check ELISA (Code. KOG-200SE), ELISA: 450nm, 96 wells Highly Sensitive 8-OHdG Check ELISA (Code. KOG-HS10E), ELISA: 450nm, 96 wells Anti 8-OHdG monoclonal antibody (N45.1) (Code: MOG-020P / MOG-100P)	Assay range: 0.5 - 200 ng/mL Assay range: 0.125-10 ng/mL 20 µg / 100 µg of IgG	OK OK -	OK OK -	- OK OK	Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE) Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E) Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG).
DNA oxidation marker	Anti Thymidine Glycol (TG) antibody (Code: MTG-100P) Clone 2E8	100 µg of IgG	-	-	OK	DNA-specific oxidative stress marker. Specific to TG in DNA polymer.
Lipid oxidation marker	HEL (13HPODE-Lys adduct) Hexanoyl-Lysine (HEL) ELISA kit (Code: KHL-700E), ELISA: 450nm, 96 wells Anti Hexanoyl-Lysine (HEL) antibody (Code: MHL-021P) Clone 5F12	Assay range: 2 - 700 nmol/L 20 µg of IgG	OK -	OK -	OK OK	N^ε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells.
	4-HNE Anti 4-HNE monoclonal antibody (HNEJ2) (Code: MHN-020P / MHN-100P)	20 µg / 100 µg of IgG	-	-	OK	4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins.
	MDA Anti MDA monoclonal antibody (Code: MMD-030n) Clone 1F83 Malondialdehyde assay kit (TBARS) (Code: KMD-008W) Colorimetric: 400-700nm scan	30 µg of IgG Assay range: 0.08 - 10 µmol/L	- OK	- OK	OK OK	Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine). By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay.
	Isoprostane Urinary isoprostane ELISA (Code: KIP-050) ELISA: 450nm, 96wells Serum isoprostane ELISA (Code: KIP-001W) ELISA: 450nm, 96wells	Assay range: 0.05 - 100 ng/mL Assay range: 0.05 - 10 ng/mL	OK -	- OK	- OK	15-Isoprostane F_2t (also known as 8-epi-PGF_2α or 8-iso-PGF_2α) is produced by peroxidation of lipoproteins. For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed.
	Anti Acrolein (ACR) antibody (Code: MAR-020n) Clone 5F6	20 µg / of IgG	-	-	OK	Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine).
	Anti 4-hydroxy-2-hexenal (4-HHE) antibody (Code: MHH-030n) Clone HHE53	30 µg of IgG	-	-	OK	Specific to HHE-histidine Michael adduct (HHE-His).
	Anti Crotonaldehyde (CRA) antibody (Code: MCA-030n) Clone 82D3	30 µg of IgG	-	-	OK	Specific to CRA-protein aduucts.
	Anti Methylglyoxal (MG) antibody (Code: MMG-030n) Clone 3C	30 µg of IgG	-	-	OK	Specific to MG-protein adduct (argpyrimidine).
	Anti 7-ketocholesterol (7-KC) antibody (Code: MKC-020n) Clone 35A	20 µg of IgG	-	-	OK	Biomarker of cholesterol oxidation. Applicable for frozen tissue only.
	Mitochondria Lipid peroxide probe (mitochondria) (Code: PLP-466J)	5 µg x3	-	-	OK	Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells.
	Lipid peroxide probe (DPPP) (Code: PLP-350J)	10 mg	-	OK	OK	Fluorescence probe for lipid peroxides. For HPLC application.
Protein oxidation marker	Dityrosine (Tyr dimer) Dityrosine (DT) ELISA [unavailable] (Code: KDT-010E), ELISA: 450nm, 96 wells Anti Dityrosine (DT) antibody (Code: MDT-020P) Clone 1C3	Assay range: 0.05-12 µmol/L 20 µg of IgG	OK -	OK -	- OK	New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours.
	Anti Dibromo-Tyrosine (DiBrY) antibody (Code: MBY-020P) Clone 3A5	20 µg of IgG	-	-	OK	Inflammation-related protein oxidation marker.
	NO-modification Anti Nitrotryptophan (NW) antibody (Code: MNW-020P) Clone 117C	20 µg of IgG	-	-	OK	A novel nitrosative stress marker derived from peroxynitrite and tryptophan.
	Nitrotyrosine ELISA (Code: KNT-002W) 96 wells	2 - 1500 nmol/L	-	OK	Δ
Anti- oxidant assay	Total Antioxidant Test kit for Potential Antioxidant(PAO), 96 wells (Code: KPA-050) Colorimetric: 480-492 nm TAC Peroxyl assay, 96 wells (Code: KPR-005W) Luminescence	Assay range: 21.9- 4378 µmol/L Assay range: 0.5～30 µmol/mL	- -	OK OK	OK OK	Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants. KPR-005W can detect peroxyl radicals(ROO·) scavenging activity.
Anti- oxidant assay	GSH Total Glutathione assay kit 100 tests (Code: KGS-419J) Colorimetric: 405-415 nm	Assay range: 5-100 µmol/L	-	-	OK	The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria.
Enzyme assay	SOD SOD activity assay kit (WST-1 method) (Code: KSD-311J) Colorimetric: 450 nm, 500 tests SOD activity assay kit (Hematoxylin method) (Code: KSD-005W) Colorimetric: 560 nm, 96 wells Cu/Zn-SOD ELISA (Human SOD1) (Code: KSE-040W) ELISA: 450 nm, 96 wells Mn-SOD ELISA (Human SOD2) (Code: KSG-025W) ELISA: 450 nm, 96 wells ec-SOD ELISA (Human SOD3) (Code: KSF-002W) ELISA: 450 nm, 96 wells	Assay range: 5-60 U/mL Assay range: 0.04 - 5 ng/mL Assay range: 25 - ng/mL Assay range: 2 - 168 ng/mL	- - - - -	OK Eryth- rocyte OK - -	OK OK OK OK OK	Can be applied to all animals, plants and bacteria. SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum. Mn-SOD(SOD2) is detectable in Mitochondria.
	CAT Catalase activity assay kit 96 wells (Code: KCA-003W) UV: 240 nm	Assay range: 6-150 U/mL	-	Eryth- rocyte	OK
	MPO activity assay kit (Code: KMP-008W) Colorimetric: 412 nm 96 microplate assays or 30 cubette assays	Assay range: 8- 400 U/mL	-	OK	OK
	GSH metabolism Glutathion Peroxidase (GPx) assay kit 192 wells (Code: KGX-005W) Colorimetric: 340 nm Glutathion Reductase (GR) assay kit 192 wells (Code: KGR-015W) Colorimetric: 340 nm	Assay range: 5-60 mU/mL Assay range: 1.5-20 mU/mL	- -	Blood Blood	OK OK
Redox regulation markers	Thioredoxin Thioredoxin I ELISA (human) 96 wells (Code: KRX-004W) ELISA: 450 nm	Assay range: 0.4- 25 ng/mL	-	OK	Δ
	Thioredoxin reductase I ELISA (human) 96 wells (Code: KRR-004W) ELISA: 450 nm	Assay range: 0.4- 25 ng/mL	-	OK	Δ
	Peroxiredoxin I ELISA (human) 96 wells (Code: KPX-005W) ELISA: 450 nm	Assay range: 0.5 - 32 ng/mL	-	OK	Δ
	Peroxiredoxin III ELISA (human) 96 wells (Code: KPZ-002W) ELISA: 450 nm	Assay range: 2 - 32 ng/mL	-	OK	Δ
Trace elements assay	Fe Iron assay kit (Ferrozine) 100 tests (Code: CFE-005) Colorimetric: 540-580 nm Iron assay kit (Nitroso-PSAP) 100 tests (Code: CFE-010) Colorimetric: 740-760 nm UIBC assay kit (Bathophenanthroline) 100 tests (Code: CBC-800) Colorimetric: 540-550 nm	Assay range: 5-1,000 µg/dL Assay range: 10-1,000 µg/dL Assay range: 10-800 µg/dL	OK OK -	OK OK OK	OK OK -	Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples. Detection of unsaturated iron binding capacity(UIBC). Species independent.
	Cu Copper assay kit (3,5-DiBr-PAESA) 100 tests (Code: CCU-400) Colorimetric: 570-590 nm	Assay range: 3-400 µg/dL	OK	OK	OK	Can detect total (free and ceruloplasmin - bond) copper. Also applicable to extractes from tissues, plants and hair.
	Zn Zink assay kit (5-Br-PAPS) (Code: CZN-001) Colorimetric: 550-580 nm	Assay range: 4-1,000 µg/dL	OK	OK	OK	Can detect total (free and protein - bond) zinc. Also applicable to extractes from tissues, plants and hair.
	Ca Calcium assay kit (Chlorophosphonazo III) (Code: CCA-020) Colorimetric: 680-700 nm, 200 tests Calcium assay kit (o-Cresolphtalein-Complexone) (Code: CCA-030) Colorimetric: 560-590 nm, 200 tests	Assay range: 0.05-20 mg/dL Assay range: 0.2-30 mg/dL	OK OK	OK OK	OK OK	Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.
	Mg Magnesium assay kit (Xyridil Blue-I) 100 tests (Code: CMG-035) Colorimetric: 660 nm	Assay range: 0.05-3.5 mg/dL	OK	OK	OK	Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.

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