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| Suitable for immunohistocheistry and western blotting. For research use only. |
| About 4-hydroxy-2-nonenal (4-HNE) |
Structure of 4-hydroxy-2-nonenal |
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies,
and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are
formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of
omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body
is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of
lipid peroxidation.
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High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous
epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999) |
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| Specifications |
| Clone #: |
HNEJ-2 |
| Immunogen: |
4-HNE-modified Keyhole Limpet Hemocyanin |
| Subclass: |
Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified. |
| Form: |
Lyophilized Powder (containing 20 µg or 100 µg of IgG) |
| Specificity: |
This antibody show almost negligible reactivity with proteins that were treated with other
aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde. |
| Applications: |
Immunohistochemistry and western blotting. |
| Storage: |
Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature. |
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| References |
| Immunohistochemistry: |
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney:
independence of tubular necrosis and sulfhydryl depletion.
Lab. Invest. 77(2), p145-155 (1997) |
| Western blotting: |
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995) |
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