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| Suitable for immunohistocheistry and ELISA. For research use only. |
| A new biomarker for lipid peroxidation |
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Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some
diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported
as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic
acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine
residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid.
This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry
and western blotting.
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Cisplatin-treated rat kidney.
(Dr. Sugiyama, Tottori Univ.) |
Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito) |
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| Specifications |
| Clone #: |
5F12 |
| Subclass: |
IgG1 kappa |
| Antigen: |
N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH) |
| Specificity: |
Specific to Hexanoyl-Lys adducts. |
| Form: |
Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950.
100 µg/mL of IgG. |
| Application: |
Immunohistochemistry
(Recommended antibody concentration is 2 µg/mL on paraformaldehyde fixed tissue), and ELISA |
| Storage: |
Less than -20°C |
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| References |
| 1) |
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa,
Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide.
J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE. |
| 2) |
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T,
Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects
of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle.
Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody. |
| 3) |
Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M,
Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein
in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions. |
| 4) |
Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E,
A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells.
Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs). |
| 5) |
Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K:
Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells.
Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse. |
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| Product name |
Code |
Specifications |
Application |
NOTICE |
| Anti HEL monoclonal antibody(clone 5F12) |
MHL-021P |
20 µg of IgG |
Immunohistochemistry |
Current product |
| Anti HEL monoclonal antibody(clone 5H4) |
MHL-020P |
20 µg of IgG |
Immunohistochemistry |
Discontinued |
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Made in Japan.
IMPORTANT NOTICE
The clone of anti HEL monoclonal antibody has been changed from 5H4 (code. MHL-020P) to 5F12
(code. MHL-021P). If you need old product, please contact our technical support.
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility.
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