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| Suitable for immunohistocheistry, western blotting and ELISA. For research use only. |
| A novel biomarker for protein oxidation/nitration: |
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Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis,
inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent
biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide
(O2-). Peroxinitrite can induce nitration of aromatic amino acids such as
Tyr, Trp, Phe and His, which results in modulation of protein functions.
To date, 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major
ONOO--modified amino acids, and growing evidences
suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress.
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An anti-6-nitrotryptophan monoclonal antibody has been developed by Prof. Yamakura at Juntendo University,
which is suitable for immunohistochemistry, western blotting and ELISA. This antibody may provide a powerful
tool for the investigation of nitrosative stress inside living bodies.
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| Specifications |
| Antigen: |
6-nitrotryptophan |
| Form: |
Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG. |
| Subclass: |
Mouse IgG1(kappa), clone 117C. Protein A purified. |
| Specificity: |
Specific for 6-nitrotryptophan |
| Application: |
Immunohistochemistry, western blotting and ELISA |
| Storage: |
Less than -20°C |
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| Reference |
| 1) |
Ikeda K, Yukihiro Hiraoka B, Iwai H, Matsumoto T, Mineki R, Taka H,
Takamori K, Ogawa H, Yamakura F:
Detection of 6-nitrotryptophan in proteins by Western blot analysis and its application for peroxynitrite-treated PC12
cells.
Nitric Oxide 16(1):18-28(2007) |
| 2) |
Degendorfer G, Chuang CY, Hammer A, Malle E, Davies MJ.
Peroxynitrous acid induces structural and functional modifications to basement membranes and its key component, laminin.
Free Radic Biol Med 89,p721-733(2015). doi: 10.1016/j.freeradbiomed.2015.09.018.
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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility.
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